Journal: PLOS ONE

Article Title: Clinical outcomes and anti-inflammatory mechanisms predict maximum heart rate improvement after physical activity training in individuals with psychiatric disorders and comorbid obesity

doi: 10.1371/journal.pone.0313759

Figure Lengend Snippet: MHR: maximum heart rate, HRSD: Hamilton rating scale for depression, PANSS-P: positive and negative syndrome scale (positive subscale), PANSS-G: positive and negative syndrome scale (general subscale), IL-10: interleukin 10, TNF-α: tumor necrosis factor alpha, ROS: reactive oxygen species; ΔΨm: mitochondrial membrane potential, VCAM: vascular cellular adhesion molecule, LEPMN: leukocyte-endothelium adhesion polymorphonuclear, RVPMN: rolling velocity polymorphonuclear cells, PSEL: P-selectin; MPO: myeloperoxidase.

Article Snippet: In total, 100 000 cells were plated in each well of 96-well plates and incubated for 30 min at 37°C with the corresponding fluorochromes, as follows: dichlorofluorescein diacetate to measure reactive oxygen species (ROS) production (485 nm excitation, 535 nm emission), MitoSOX to measure mitochondrial ROS (mROS) (510 nm excitation, 580 nm emission), tetramethylrodamin methyl ester to assess mitochondrial membrane potential (ΔΨm) (552 nm excitation, 574 nm emission), superoxide dismutase (SOD) (total SOD/T-SOD Activity Assay Kit–Colorimetric—from NOVUS), and 5-chloromethylfluorescein diacetate to measure intracellular glutathione (GSH) (492 nm excitation, 517 nm emission).

Techniques: Membrane

Journal: Antioxidants

Article Title: Synthetic Cyclic C 5 -Curcuminoids Increase Antioxidant Defense and Reduce Inflammation in 6-OHDA-Induced Retinoic Acid-Differentiated SH-SY5Y Cells

doi: 10.3390/antiox14091057

Figure Lengend Snippet: Determination of ROS production and total antioxidant capacity in 6-OHDA-induced differentiated SH-SY5Y cells. ROS determination was carried out using a Fluorometric Intracellular ROS Kit. ROS levels were compared to the control cells and expressed as a percentage. The TAC levels were determined using an Antioxidant Capacity Assay Kit. ( A ) Intracellular ROS levels in 6-OHDA-induced differentiated SH-SY5Y cells after treatment with sample compounds and rasagiline. ( B ) Total antioxidant capacity of 6-OHDA-induced differentiated SH-SY5Y cells after treatment with sample compounds and rasagiline. Each column shows the mean ± SD derived from three independent experiments ( n = 3). The * signifies a p -value of less than 0.05 in comparison to the control. The † indicates p < 0.05, compared with the 6-OHDA treatment. Abbreviations: 6-OHDA, 6-hydroxydopamine; ROS, reactive oxygen species.

Article Snippet: The amount of ROS was determined in SH-SY5Y cells using a Fluorometric Intracellular ROS Kit (Merck Life Sciences Kft., Budapest, Hungary).

Techniques: Control, Derivative Assay, Comparison